CORO1A

CORO1A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2AKF, 2AQ5, 2B4E
Identifiers
Aliases	CORO1A, CLABP, CLIPINA, HCORO1, IMD8, TACO, p57, coronin 1A
External IDs	OMIM: 605000; MGI: 1345961; HomoloGene: 6545; GeneCards: CORO1A; OMA:CORO1A - orthologs
Gene location (Human) Chr. Chromosome 16 (human)[1] Band 16p11.2 Start 30,182,827 bp[1] End 30,189,076 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 69.25 cM|7 F3 Start 126,298,945 bp[2] End 126,306,959 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in granulocyte monocyte blood spleen appendix bone marrow bone marrow cells lymph node trabecular bone thymus Top expressed in granulocyte mesenteric lymph nodes thymus spleen tibiofemoral joint blood bone marrow right lung lobe dentate gyrus of hippocampal formation granule cell prefrontal cortex More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding protein C-terminus binding identical protein binding phosphatidylinositol 3-kinase binding protein homodimerization activity actin monomer binding myosin heavy chain binding cytoskeletal protein binding actin filament binding actin binding Cellular component phagocytic cup extracellular exosome cytoplasm actin cytoskeleton cell cortex nucleus cell leading edge axon lamellipodium cortical actin cytoskeleton early endosome cell-cell junction membrane actin filament phagocytic vesicle membrane plasma membrane cytoplasmic vesicle immunological synapse cytoskeleton phagocytic vesicle cytosol Biological process negative regulation of vesicle fusion calcium ion transport homeostasis of number of cells within a tissue negative regulation of actin nucleation phagocytosis response to cytokine nerve growth factor signaling pathway T cell homeostasis positive regulation of cell migration regulation of actin cytoskeleton organization positive chemotaxis actin filament organization positive regulation of T cell proliferation cellular response to interleukin-4 natural killer cell degranulation regulation of release of sequestered calcium ion into cytosol regulation of actin polymerization or depolymerization cell-substrate adhesion regulation of actin filament polymerization negative regulation of neuron apoptotic process positive regulation of T cell activation regulation of cell shape innate immune response early endosome to recycling endosome transport cell migration actin cytoskeleton organization uropod organization immunological synapse formation leukocyte chemotaxis phagolysosome assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 11151 12721 Ensembl ENSG00000102879 ENSMUSG00000030707 UniProt P31146 O89053 RefSeq (mRNA) NM_001193333 NM_007074 NM_001301374 NM_009898 RefSeq (protein) NP_001180262 NP_009005 NP_001288303 NP_034028 Location (UCSC) Chr 16: 30.18 – 30.19 Mb Chr 7: 126.3 – 126.31 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Coronin-1A is a protein that in humans is encoded by the CORO1A gene.^[5][6] It has been implicated in both T-cell mediated immunity and mitochondrial apoptosis. In a recent genome-wide longevity study, its expression levels were found to be negatively associated both with age at the time of blood sample and the survival time after blood draw.^[7]

Discovery

The coronin protein family was discovered in 1991 by Eugenio L. Hostos. Hostos used a cytoskeletal preparation called the “contracted propeller” that efficiently helped with the purification of cytoskeletal proteins. This technique allowed him to precipitate actomyosin components together with the desired proteins.^[8]

These protein were named Corona, which is the Latin word for crown, because of the crown-like shape that it forms when making contact with the surface of the cell. Coronin-1a has been the most researched one due to its complexity and intriguing structural components. After research, it was determined that coronin-1a serves as actin binding facilitator when reacted with K-glutamate. The anion K + and glutamate were used because of it similarity to the environment inside the cell, allowing coronin-1a to bind to F-actin.

Later on, the complementary DNA (cDNA) of coronin-1a was cloned in an expression library, this led to the conclusion that coronin-1a has very similar structure to the beta (β) subunits of the G proteins (Gβ). Therefore, it was established that coronin-1a has five WD motif repeats, and this repeats seven times forming a propeller like structure.^[8]

In the cell, coronin-1a serves as an auxiliary to many cytoskeletal process that involve actin. It was concluded that coronin-1a is known to affect the “cytoskeletal reorganization” as well “actin dynamics” together with other protein. ^[8]

Phylogeny

The coronin family is composed of twelve subfamilies which include: seven subfamilies that fall under vertebrates and five subfamilies that are composed of metazoans, fungi and amoeba.

The evolutionary coronin subfamilies have been grouped by its similarities and relationships between the different proteins. Coronin-1a (also referenced as CORO1A, Coronin 4 and CRN4) has been found in 19 vertebrates.^[9]

Function

Coronin-1a has been found in the cell cortex of macrophages, which are white blood cells, helping with a process called phagocytosis. The model on Figure 3 shows coronin-1a's involvement in macrophages. When the cell is at rest, Coronin-1a is spread out throughout the cytoplasm and the cell cortex. Therefore, when a pathogen enters the cell, Coronin-1a binds to phagosomal membrane making sure of the binding and activation of calcineurin, this resulting in a stop of fusion lysosomes with phagosomes. In other words, if coronin-1a is removed and calcineurin is inhibited then it allows the initiation of the fusion of phagosomes with lysosome and the killing of mycobacteria.^[10]

The phylogenetic tree of the coronin family it is broad. The same way that coronin-1a helps with the reorganization of the cytoskeleton and dynamic activity with other proteins in vertebrates, Coronin can also be seen in non-vertebrates, for example in Toxoplasma gondii (also known as TgCor).^[11]

Toxoplasma gondii coronin (TgCor) binds to F-actin and it accelerates the actin polymerization process. It also prevents incursions and exits. As well as every other coronin, TgCor is an actin binding protein, it delocalizes to the posterior side of invading parasites and blocks them from leaving.^[11]

Structure

The structure of coronin-1A is made out of five WD repeats, and this motifs repeat seven time forming a propeller like structures.

The new ribbon visualization of the secondary structure of coronin-1a. In model A, is the front view of coronin-1a, the secondary structure allows you to clearly see the parallel beta sheets moving towards the bottom of the structure. Model B, is the side view of the protein which shows the turns and the coils between the beta sheets. From this pictures we are able to see that the alpha helix and helix strands are concentrated at the bottom of the protein.^[12]

Coronin-1a was input into Database of Secondary Structure Program, where the Protein Data Bank database entered and a secondary structure panel is designed where one is clearly able to see the seven repeat that makes the propeller. Also, it displays the amino acid sequence of coronin-1a. The yellow arrows mean the beta strands, the purple loops are the turns, the black lines means empty meaning that there was no secondary structure assigned, the light pink is 3/10-helix is formed, royal blue line is a bend and finally the red helix signifies the alpha helices.

References

External links

• Human CORO1A genome location and CORO1A gene details page in the UCSC Genome Browser.
• Overview of all the structural information available in the PDB for UniProt: O89053 (Coronin-1A) at the PDBe-KB.

Further reading