UGT8

Protein-coding gene in the species Homo sapiens

UGT8

Identifiers

Aliases

UGT8, CGT, UGT4, UDP glycosyltransferase 8, GalCer Synthase, Ceramide Galactosyltransferase

External IDs

OMIM: 601291; MGI: 109522; HomoloGene: 20715; GeneCards: UGT8; OMA:UGT8 - orthologs

Gene location (Human)

Chr.	Chromosome 4 (human)^[1]

Band	4q26	Start	114,598,770 bp^[1]
Band	4q26	End	114,678,225 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 3 (mouse)^[2]

Band	3\|3 G1	Start	125,658,920 bp^[2]
Band	3\|3 G1	End	125,732,268 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

inferior ganglion of vagus nerve

corpus callosum

subthalamic nucleus

superior vestibular nucleus

C1 segment

pons

external globus pallidus

pars reticulata

ventral tegmental area

Pars compacta

Top expressed in

deep cerebellar nuclei

pontine nuclei

ventral tegmental area

lateral geniculate nucleus

globus pallidus

dorsal tegmental nucleus

substantia nigra

lateral hypothalamus

medial geniculate nucleus

medial vestibular nucleus

More reference expression data

BioGPS

n/a

Gene ontology
Molecular function	hexosyltransferase activity glycosyltransferase activity glucuronosyltransferase activity transferase activity N-acylsphingosine galactosyltransferase activity UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity UDP-glycosyltransferase activity
Cellular component	integral component of membrane membrane intracellular membrane-bounded organelle plasma membrane
Biological process	lipid metabolism neuron projection morphogenesis galactosylceramide biosynthetic process glycosphingolipid metabolic process peripheral nervous system development central nervous system development paranodal junction assembly metabolism protein localization to paranode region of axon cytoskeleton organization sphingolipid metabolic process
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


7368


22239

Ensembl


ENSG00000174607


ENSMUSG00000032854

UniProt


Q16880


Q64676

RefSeq (mRNA)


NM_001128174 NM_003360 NM_001322112 NM_001322113 NM_001322114


NM_011674

RefSeq (protein)


NP_001121646 NP_001309041 NP_001309042 NP_001309043 NP_003351


NP_035804

Location (UCSC)

Chr 4: 114.6 – 114.68 Mb

Chr 3: 125.66 – 125.73 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

2-hydroxyacylsphingosine 1-beta-galactosyltransferase is an enzyme that in humans is encoded by the UGT8 gene.^[5]^[6]

Function

Galactocerebrosides are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system and are also present in small amounts in kidney. The key enzymatic step in the biosynthesis of galactocerebrosides consists of the transfer of galactose to ceramide catalyzed by UDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45). The enzyme encoded by the CGT gene is the first involved in complex lipid biosynthesis in the myelinating oligodendrocyte.^[6]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000174607 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000032854 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Bosio A, Binczek E, Le Beau MM, Fernald AA, Stoffel W (Dec 1996). "The human gene CGT encoding the UDP-galactose ceramide galactosyl transferase (cerebroside synthase): cloning, characterization, and assignment to human chromosome 4, band q26". Genomics. 34 (1): 69–75. doi:10.1006/geno.1996.0242. PMID 8661025.
^ ^a ^b "Entrez Gene: UGT8 UDP glycosyltransferase 8 (UDP-galactose ceramide galactosyltransferase)".

Yahi N, Spitalnik SL, Stefano KA, De Micco P, Gonzalez-Scarano F, Fantini J (1994). "Interferon-gamma decreases cell surface expression of galactosyl ceramide, the receptor for HIV-1 GP120 on human colonic epithelial cells". Virology. 204 (2): 550–7. doi:10.1006/viro.1994.1568. PMID 7941321.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Bhat S, Mettus RV, Reddy EP, Ugen KE, Srikanthan V, Williams WV, Weiner DB (1993). "The galactosyl ceramide/sulfatide receptor binding region of HIV-1 gp120 maps to amino acids 206-275". AIDS Res. Hum. Retroviruses. 9 (2): 175–81. doi:10.1089/aid.1993.9.175. PMID 8457384.
Kapitonov D, Yu RK (1997). "Cloning, characterization, and expression of human ceramide galactosyltransferase cDNA". Biochem. Biophys. Res. Commun. 232 (2): 449–53. doi:10.1006/bbrc.1997.6240. PMID 9125199.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Sprong H, Kruithof B, Leijendekker R, Slot JW, van Meer G, van der Sluijs P (1998). "UDP-galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum". J. Biol. Chem. 273 (40): 25880–8. doi:10.1074/jbc.273.40.25880. PMID 9748263.
Iida A, Saito S, Sekine A, Mishima C, Kitamura Y, Kondo K, Harigae S, Osawa S, Nakamura Y (2002). "Catalog of 86 single-nucleotide polymorphisms (SNPs) in three uridine diphosphate glycosyltransferase genes: UGT2A1, UGT2B15, and UGT8". J. Hum. Genet. 47 (10): 505–10. doi:10.1007/s100380200075. PMID 12376738.
Sprong H, Degroote S, Nilsson T, Kawakita M, Ishida N, van der Sluijs P, van Meer G (2003). "Association of the Golgi UDP-galactose transporter with UDP-galactose:ceramide galactosyltransferase allows UDP-galactose import in the endoplasmic reticulum". Mol. Biol. Cell. 14 (8): 3482–93. doi:10.1091/mbc.E03-03-0130. PMC 181583. PMID 12925779.
Tencomnao T, Kapitonov D, Bieberich E, Yu RK (2004). "Transcriptional regulation of the human UDP-galactose:ceramide galactosyltransferase (hCGT) gene expression: functional role of GC-box and CRE". Glycoconj. J. 20 (5): 339–51. doi:10.1023/B:GLYC.0000033630.58533.16. PMID 15229398. S2CID 38983312.

Transferases: glycosyltransferases (EC 2.4)

2.4.1: Hexosyl-
transferases

Glucosyl-	Phosphorylase Starch Glycogen Cellobiose Myo- Glycogen synthase Debranching enzyme Branching enzyme 1,3-Beta-glucan synthase Ceramide glucosyltransferase N-glycosyltransferase
Galactosyl-	Lactose synthase B-N-acetylglucosaminyl-glycopeptide b-1,4-galactosyltransferase Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase (C1GALT1)
Glucuronosyl-	B3GAT1 B3GAT2 B3GAT3 UGT1A1 UGT1A3 UGT1A4 UGT1A5 UGT1A6 UGT1A7 UGT1A8 UGT1A9 UGT1A10 UGT2A1 UGT2A2 UGT2A3 UGT2B4 UGT2B7 UGT2B10 UGT2B11 UGT2B15 UGT2B17 UGT2B28 Hyaluronan synthase: HAS1 HAS2 HAS3
Fucosyl-	POFUT1 POFUT2 FUT1 FUT2 FUT3 FUT4 FUT5 FUT6 FUT7 FUT8 FUT9 FUT10 FUT11
Mannosyl-	Dolichyl-phosphate-mannose-protein mannosyltransferase POMT1 POMT2 DPM1 DPM3 ALG1 ALG2 ALG3 ALG6 ALG8 ALG9 ALG12

2.4.2: Pentosyl-
transferases

Ribose

ADP-ribosyltransferase	NAD⁺:diphthamide ADP-ribosyltransferase Diphtheria toxin Pseudomonas exotoxin NAD(P)⁺:arginine ADP-ribosyltransferase Pertussis toxin Cholera toxin Poly ADP ribose polymerase Sirtuin
Phosphoribosyltransferase	Adenine phosphoribosyltransferase Hypoxanthine-guanine phosphoribosyltransferase Uracil phosphoribosyltransferase Amidophosphoribosyltransferase
Other	Purine nucleoside phosphorylase: Thymidine phosphorylase ECGF1

Other

2.4.99: Sialyl
transferases

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)